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cd105 antibody (sn6, pe  (Thermo Fisher)


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    Thermo Fisher cd105 antibody (sn6, pe
    Cd105 Antibody (Sn6, Pe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd105+antibody+(sn6%2C+pe/pm39815324-96-2-6
    Average 90 stars, based on 1 article reviews
    cd105 antibody (sn6, pe - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Doxorubicin Activity Is Modulated by Traditional Herbal Extracts in a 2D and 3D Multicellular Sphere Model of Leukemia.
    Article Snippet: MSC phenotype was confirmed by evaluating antigens as: CD34 (anti CD34-APC, clone 581, Thermo Scientific®, Waltham, MA, USA), CD45 (anti CD45-PerCP, clone 2D1, BD Biosciences®), CD73 (anti CD73-FITC, clone AD2, BD PharmingenTM, San Diego, CA, USA) and CD105 (anti CD105-PE, clone SN6, Thermo Scientific®) by flow cytometry using the FACSAria II-U cytometer (BD Biosciences®) and the data were analyzed using FlowJo 10.8.1 Software (Tree star, Ashland, OR, USA).

    Article Title: A Novel FACS-Based Workflow for Simultaneous Assessment of RedOx Status, Cellular Phenotype, and Mitochondrial Genome Stability
    Article Snippet: Anti-CD105-PE (eBiosciences, San Diego, CA, USA; clone SN6) was used to determine MSC phenotype.

    Article Title: Doxorubicin Activity Is Modulated by Traditional Herbal Extracts in a 2D and 3D Multicellular Sphere Model of Leukemia
    Article Snippet: MSC phenotype was confirmed by evaluating antigens as: CD34 (anti CD34-APC, clone 581, Thermo Scientific ® , Waltham, MA, USA), CD45 (anti CD45-PerCP, clone 2D1, BD Biosciences ® ), CD73 (anti CD73-FITC, clone AD2, BD PharmingenTM, San Diego, CA, USA) and CD105 (anti CD105-PE, clone SN6, Thermo Scientific ® ) by flow cytometry using the FACSAria II-U cytometer (BD Biosciences ® ) and the data were analyzed using FlowJo 10.8.1 Software (Tree star, Ashland, OR, USA).

    Article Title: MSC-Derived Small Extracellular Vesicles Attenuate Autoimmune Dacryoadenitis by Promoting M2 Macrophage Polarization and Inducing Tregs via miR-100-5p
    Article Snippet: The phenotype of hUC-MSCs was determined by flow cytometry using specific antibodies as follows: CD29-FITC (clone TS2/16), CD34-FITC (clone 4H11), CD44-FITC (clone IM7), CD45-FITC (clone HI30) and CD105-PE (clone SN6) (eBioscience, USA); CD73-FITC (clone AD2), CD90-FITC (clone 5E10), CD11b-FITC (clone M1/70) and HLA-DR-PE (clone LN3) (Biolegend, USA).

    Staining:

    Article Title: Identification and characterization of tumor and stromal derived liquid biopsy analytes in pancreatic ductal adenocarcinoma.
    Article Snippet: Rabbit anti-mouse (Alexa FluorTM 488, Invitrogen, A-11059, 1:200) and goat anti-rabbit (Alexa FluorTM 488, Invitrogen, A-11008, 1:200) were used as a secondary antibody and incubated for 60 min. .. Directly conjugated CD105 antibody (Sn6, PE, eBioscienceTM, Invitrogen, 1:200), CD45 antibody (clone H130, PerCP, BioLegend, 1:200) and DRAQ5TM for nuclear staining (Cell Signaling, 1:1000) were incubated for 60 min at room temperature. .. For the staining of the enriched cells after ParsortixTM cell separation the CD105 antibody was replaced by the pan-cytokeratin antibody (AE1/AE3, eFluorTM 570, Invitrogen, 1:200).

    Incubation:

    Article Title: Identification and characterization of tumor and stromal derived liquid biopsy analytes in pancreatic ductal adenocarcinoma.
    Article Snippet: Rabbit anti-mouse (Alexa FluorTM 488, Invitrogen, A-11059, 1:200) and goat anti-rabbit (Alexa FluorTM 488, Invitrogen, A-11008, 1:200) were used as a secondary antibody and incubated for 60 min. .. Directly conjugated CD105 antibody (Sn6, PE, eBioscienceTM, Invitrogen, 1:200), CD45 antibody (clone H130, PerCP, BioLegend, 1:200) and DRAQ5TM for nuclear staining (Cell Signaling, 1:1000) were incubated for 60 min at room temperature. .. For the staining of the enriched cells after ParsortixTM cell separation the CD105 antibody was replaced by the pan-cytokeratin antibody (AE1/AE3, eFluorTM 570, Invitrogen, 1:200).

    Purification:

    Article Title: 3D bioprinting of multilayered scaffolds with spatially differentiated ADMSCs for rotator cuff tendon-to-bone interface regeneration
    Article Snippet: .. We used the following primary antibodies: CD34 (MEC14.7, phycoerythrin (PE), BioLegend), CD44 (W4/86, purified, Bio-Rad), CD45 (L12/201, purified, Bio-Rad), CD51 (23C6, purified, BioLegend), CD90 (OX-7, PE, BioLegend), CD90 (5E10, purified, BioLegend), CD105 (SN6, PE, Invitrogen), rat IgG2a isotype (RTK2758, PE, BioLegend), mouse IgG1 isotype (MOPC-21, purified, BioLegend), and mouse IgG1 isotype (MOPC-21, PE, BioLegend). .. A secondary antibody, anti-mouse IgG1 (RMG1–1, PE, BioLegend), was used following the primary antibodies of CD44, CD45, CD51, CD90 clone 5E10, and purified mouse IgG1 isotype.



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    Image Search Results


    Antibodies used in this manuscript.

    Journal: Research Square

    Article Title: A Mutant Methionyl-tRNA Synthetase-Based toolkit to assess induced-Mesenchymal Stromal Cell secretome in mixed-culture disease models

    doi: 10.21203/rs.3.rs-2838195/v1

    Figure Lengend Snippet: Antibodies used in this manuscript.

    Article Snippet: CD105 (Endoglin) Monoclonal Antibody (SN6), PE , eBioscience (12-1057-41) , 1:20 (FC).

    Techniques: Immunocytochemistry, Western Blot, Flow Cytometry, Functional Assay, Antibody Labeling

    MetRS L274G -expressing iPSCs were differentiated to iMSCs. iMSC identity was validated by (A) expression of CD73, CD90, and CD105, and lack of expression of CD45, CD34, CD11b, CD79a, and HLA-DR, and (B) multipotency demonstrated by differentiation to adipocytes (mFABP4), osteoblasts (hOsteocalcin), and chondrocytes (Alcian Blue 8GX). C) Reverse transcription PCR with species-specific Taqman probe sets was used to confirm continued expression of MetRS L274 (Mars) in iMSCs following differentiation and expansion. Functionality of MetRS L274G was confirmed by FUNCAT labeling with Cy5-DBCO in cells cultured in ANL-supplemented media.

    Journal: Research Square

    Article Title: A Mutant Methionyl-tRNA Synthetase-Based toolkit to assess induced-Mesenchymal Stromal Cell secretome in mixed-culture disease models

    doi: 10.21203/rs.3.rs-2838195/v1

    Figure Lengend Snippet: MetRS L274G -expressing iPSCs were differentiated to iMSCs. iMSC identity was validated by (A) expression of CD73, CD90, and CD105, and lack of expression of CD45, CD34, CD11b, CD79a, and HLA-DR, and (B) multipotency demonstrated by differentiation to adipocytes (mFABP4), osteoblasts (hOsteocalcin), and chondrocytes (Alcian Blue 8GX). C) Reverse transcription PCR with species-specific Taqman probe sets was used to confirm continued expression of MetRS L274 (Mars) in iMSCs following differentiation and expansion. Functionality of MetRS L274G was confirmed by FUNCAT labeling with Cy5-DBCO in cells cultured in ANL-supplemented media.

    Article Snippet: CD105 (Endoglin) Monoclonal Antibody (SN6), PE , eBioscience (12-1057-41) , 1:20 (FC).

    Techniques: Expressing, Reverse Transcription, Labeling, Cell Culture

    Data on antibodies and their dilutions used for FACS analysis in the study.

    Journal: Frontiers in Veterinary Science

    Article Title: Comparison of Canine and Feline Adipose-Derived Mesenchymal Stem Cells/Medicinal Signaling Cells With Regard to Cell Surface Marker Expression, Viability, Proliferation, and Differentiation Potential

    doi: 10.3389/fvets.2020.610240

    Figure Lengend Snippet: Data on antibodies and their dilutions used for FACS analysis in the study.

    Article Snippet: CD105 , PE-Cy7 , SN6 , Mouse IgG1 , Human , 25-1057-42 , eBioscience, USA , / , /.

    Techniques: Marker, Conjugation Assay

    Human UCM and BM MSCs single stained with antibodies against surface markers. MSCs were CD14-CD19-CD34-CD45-CD73+CD90+ and CD105+, correlating with an MSC phenotype defined by ISCT. Grey: unstained; black: stained.

    Journal: Stem Cells International

    Article Title: Osteogenic Potential of Human Umbilical Cord Mesenchymal Stem Cells on Coralline Hydroxyapatite/Calcium Carbonate Microparticles

    doi: 10.1155/2018/4258613

    Figure Lengend Snippet: Human UCM and BM MSCs single stained with antibodies against surface markers. MSCs were CD14-CD19-CD34-CD45-CD73+CD90+ and CD105+, correlating with an MSC phenotype defined by ISCT. Grey: unstained; black: stained.

    Article Snippet: The following antibodies were used to phenotype the cells based on the International Society for Cellular Therapy (ISCT) criteria [ ]: CD14-APC-eFluor780 (clone 61D3), CD34-eFluor450 (clone 4H11), CD73-FITC (clone AD2), CD90-APC (clone eBio5E10), CD105-PE (clone SN6) (eBioscience, Hatfield, Ireland, UK), CD19-PE-Cy7 (clone J3-119), and CD45-Krome Orange (clone J.33) (Beckman Coulter, High Wycombe, UK).

    Techniques: Staining